Description

Dr. Dushan Wadduwage from the Harvard University, United States delivered a talk about “Rapid Wide Field Multiphoton Microscopy through Scattering Tissue” in the BME Seminar organized by our chapter. Here are some of glimpses from the event. Abstract on talk : Most excitatory synapses in the mammalian brain are located on dendritic spines, making spines convenient proxies for excitatory synaptic presence. When in-vivo two-photon imaging revealed that dendritic spines are dynamic structures, their addition and elimination were interpreted as excitatory synapse gain and loss. Currently, imaging a full L2/3 dendritic arbor at synaptic resolution with a standard point scanning two photon microscope (PS-TPM) takes 60-90 minutes. Imaging rapid synaptic remodeling events on the order of minutes, across a large volume is thus not possible. To enable imaging rapid dynamic event of this nature, through scattering tissue, they are now developing a range of wide field multi photon microscopy technologies. The imaging methods are based on two key techniques temporal focusing, and computational imaging, they use temporal focusing for depth selective multiphoton excitation in wide field. Excitation light at NIR wavelengths, penetrates deep the emission photons, at visible wavelengths however encounter severe scattering. They use computational imaging to de-scatter these photons with a technique called de-scattering with excitation patterning or deep. They demonstrate deep with classical computational imaging algorithms as well as state-of-the-art deep convolutional neural networks. Their results suggest orders of magnitude faster imaging speeds, that could potentially render, neuroscience experiments that were previously prohibitively slow possible.